Table 2 Primers sequence used for qRT-PCR, source and accession number at NCBI site
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | Source | Accession no. |
|---|---|---|---|---|
Ppara | TGGAGTCCACGCATGTGAAG | CGCCAGCTTTAGCCGAATAG | Somm et al. (2009) | NM_013196.1 |
Rxra | GGACACCAAACATTTCCTGCC | GATGTGCTTGGTGAAGGA | Gillio-Meina et al. (2009) | NM_012805.2 |
Lxra | AGGAGTGTCGACTTCGCAAA | CTCTTCTTGCCGCTTCAGTTT | Cha and Repa (2007) | NM_031627.2 |
Srebf1 | GGAGCCATGGATTGCACATT | GGCCCGGGAAGTCACTGT | Cha and Repa (2007) | XM_213329.6 |
Acox1 | GGCATGTAACCCGTAGCACT | GCCCAACTGTGACTTCCATT | Baarine et al. (2012) | NM_017340.2 |
Cpt1a | CTCCGCCTGAGCCATGAAG | CACCAGTGATGATGCCATTCT | Yang et al. (2011) | NM_031559.2 |
Acadvl | ATGACCCTGCCAAGAATGAC | TCCCAGGGTAACGCTAACAC | Buettner et al. (2006) | NM_012891.2 |
Fasn | CAGGTGTGTGATGGGAAGG | CCGTACACTCACTCGAGGC | Hansmannel et al. (2006) | NM_017332.1 |
Acaca | GCCATCCGGTTTGTTGTCA | GGATACCTGCAGTTTGAGCCA | Ronnebaum et al. (2008) | NM_022193.1 |
Ncor1 | AGTCGCTACAGCCCAGAGTC | CTCCTCTCTGGGGATTTTCC | Malik et al. (2010) | XM_577103.4 |
Actb | TGTCACCAACTGGGACGATA | GGGGTGTTGAAGGTCTCAAA | Bonefeld et al. (2008) | NM_031144.3 |
Gapdh | ACCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGTA | Nelissen et al. (2010) | NM_017008.4 |
Rn18 s | ACGGACCAGAGCGAAAGCAT | TGTCAATCCTGTCCGTGTCC | Bonefeld et al. (2008) | NR_046237.1 |