- Spot number, protein ID, mean spot density ± SEM (group mean) and level of significance (P) for each protein affected by treatment is shown for n = 10 mice per group. Log transformed spot densities from aorta tissue were analysed by two-way ANOVA for the effect of fat intake, vitamin composition and interaction between these factors (P < 0.05). ANOVA with post hoc testing was carried out to establish the differential effects of folic acid, B vitamin and fat intake on protein expression. Green represents upregulated proteins and red represents downregulated proteins, respectively. 1; fat status, 2; vitamin status, 3; fat and vitamin status, 4; HF versus HF–F, 5; HF–F versus HF–F–B, where C; control, HF; high fat, F–; diet depleted of folic acid, F–B–; diet depleted of folic acid and vitamins B6 and B12
