Fig. 1

AACE gene expression in Ppara-null and WT mice fed increasing concentrations of DP/DCH. Mice were fed diets containing 6/77, 20/63 or 50/33 % DP/DCH during 8 days. a Heat maps of AACE gene expression in the livers of WT mice, b Ppara-null (KO) mice, and c WT versus Ppara-null mice (n = 3–4). d Validation of gene expression results by RT-PCR analysis of Sds, e Acmsd and f Kynu in the livers of mice. g SDS expression was analyzed in total protein liver extracts of animals; representative data are shown from one mouse per lane. h Relative expression of Pck1 in Ppara-null and WT mice by RT-PCR from liver mRNA. The expression analysis was conducted after refeeding for 3 h. White bars  WT; black bars  Ppara-null. Results represent mean ± SEM (n = 4–5)