Effect of genetic polymorphisms involved in folate metabolism on the concentration of serum folate and plasma total homocysteine (p-tHcy) in healthy subjects after short-term folic acid supplementation: a randomized, double blind, crossover study

Table 1 PCR methods used in this study

Gene		Primers	Annealing temperature in PCR (°C)	Restriction enzyme	PCR product (bp)	References
MTHFR-677C>T	(S)	TGAAGGAGAAGGTGTCTGCGGGA	55	Hinf	198, 175, 23	Fredriksen et al. (2007), Charasson et al. (2009), Frosst et al. (1995), Markan et al. (2007)
	(AS)	AGGACGGTGCGGTGAGAGTG
MTHFR-1298A>C	(S)	CTTTGGGGAGCTGAAGGACTACTA	55	MboII	84, 56, 31, 30, 28, 18	Fredriksen et al. (2007), Charasson et al. (2009), Frosst et al. (1995), Markan et al. (2007)
	(AS)	CACTTTGTGACCATTCCGGTTTG
RFC1-80G>A	(S)	AGTGTCACCTTCGTCCCCTC	55	CfoI	162, 125, 68, 37	Fredrikson et al. (2007), Markan et al. (2007)
	(AS)	CTCCCGCGTGAAGTTCTT
MTHFD1-1958G>A	(S)	CACTCCAGTGTTTGTCCATG	55	MspI	267, 196, 70, 56, 8	Fredriksen et al. (2007), Charasson et al. (2009), Markan et al. (2007)
	(AS)	GCATCTTGAGAGCCCTGAC
MTR-2756A>G	(S)	TGTTCCCAGCTGTTAGATGAAAAT	55	HaeIII	211, 131, 80	Fredriksen et al. (2007), Charasson et al. (2009)
	(AS)	GATCCAAAGCCTTTTACACTCCTC
DHFR-19del	(F1)	CCACGGTCGGGGTACCTGGG	63		113, 92	Gemmati et al. (2009), Horie et al. (1995), Skibola et al. (2002), Canalle et al. (2006)
	(F2)	ACGGTCGGGGTGGCCGAC
	(R)	AAAAGGGGAATCCAGTCGG
TS	(P1)	GTGGCTCCTGCGTTTCCCCC	62			Gemmati et al. (2009), Horie et al. (1995), Skibola et al. (2002), Canalle et al. (2006)
	(P2)	CCAAGCTTGGCTCCGAGCCGG
		CCACAGGCATGGCGCGG

MTHFR methylene tetrahydrofolate reductase, RFC1 reduced folate carrier protein-1, MTHFD 1 methylenetetrahydrofolate dehydrogenase 1, MTR methionine synthase, DHFR dihydrofolate reductase, TS thymidylate synthase

ISSN: 1865-3499