Effect of genetic polymorphisms involved in folate metabolism on the concentration of serum folate and plasma total homocysteine (p-tHcy) in healthy subjects after short-term folic acid supplementation: a randomized, double blind, crossover study

Table 5 Concentration of p-tHcy associated with combined genotypes before and after supplementation with folic acid

			Baseline p-tHcy	p value for the comparison between combined polymorphisms	2 weeks p-tHcy	p value for the comparison between combined polymorphisms
	MTHFR-677C>T
MTHFR-1298A>C
AA	CC	(12)	6.6 ± 2.2*¹		6.7 ± 1.4
AC+CC	CC	(28)	7.6 ± 1.7	AA,CC–AA,CT+TT (p = 0.0003)	7.1 ± 1.4
AA	CT+TT	(34)	9.6 ± 4.8		8.7 ± 4.3
AC+CC	CT+TT	(17)	8.6 ± 2.3		7.7 ± 1.8
MTHFD1-1958G>A
GG	CC	(13)	7.7 ± 1.6*²		7.5 ± 1.3
GA+AA	CC	(27)	7.2 ± 2.1	GA+AA,CC–GA+AA, CT+TT (p = 0.004)	6.8 ± 1.4	GA+AA,CC−GA+AA,CT+TT (p = 0.002)
GG	CT+TT	(20)	8.8 ± 1.8		7.5 ± 1.4
GA+AA	CT+TT	(31)	9.6 ± 5.1		8.9 ± 4.5
MTR-2756A>G
AA	CC	(24)	7.6 ± 2.2*³		7.3 ± 1.6
AG+GG	CC	(16)	6.9 ± 1.2	AG+GG,CC–AG+GG,CT+TT (p = 0.004)	6.5 ± 0.8
AA	CT+TT	(30)	8.8 ± 2.2		8.0 ± 2.1
AG+GG	CT+TT	(21)	10.0 ± 6.2		8.9 ± 5.3
DHFR 19del
WW	CC	(18)	7.8 ± 1.8*⁴		7.1 ± 1.2
WD+DD	CC	(22)	7.0 ± 2.0	WD+DD,CC–WW,CT+TT (p = 0.002)	6.9 ± 1.6
WW	CT+TT	(16)	9.2 ± 2.4		8.3 ± 2.0
WD+DD	CT+TT	(35)	9.3 ± 4.8	WD+DD,CC–WD+DD,CT+TT (p = 0.002)	8.4 ± 4.3
TS
3R3R	CC	(12)	7.6 ± 1.8*⁵		7.0 ± 1.6
2R2R + 2R3R	CC	(28)	7.2 ± 2.0	2R2R + 2R3R,CC−2R2R + 2R3R, CT+TT	7.0 ± 1.3
3R3R	CT+TT	(11)	8.2 ± 0.8	(p = 0.008)	7.8 ± 1.5
2R2R + 2R3R	CT+TT	(40)	9.6 ± 4.7		8.5 ± 4.1
RFC1-80G>A
GG	CC	(14)	7.9 ± 2.1*⁶		7.3 ± 1.5
GA	CC	(15)	6.9 ± 1.9		6.9 ± 1.3
AA	CC	(11)	7.3 ± 1.6	GA,CC–GA,CT+TT (p = 0.001)	6.7 ± 1.5
GG	CT+TT	(12)	8.3 ± 1.7		7.5 ± 1.4
GA	CT+TT	(26)	9.8 ± 5.4		8.6 ± 4.5
AA	CT+TT	(13)	9.2 ± 2.8		8.6 ± 2.8

Data are presented as mean ± SD. Number of subjects in the parentheses. The concentration of p-tHcy is given µmol/L. ANOVA test
*¹ p = 0.002, *² p = 0.007, *³ p = 0.007, *⁴ p = 0.006, *⁵ p = 0.006, *⁶ p = 0.01

ISSN: 1865-3499