Fig. 1

Effects of DFO on Egr1 mRNA and transcriptional activity. Egr1 (a) and TfR1 (b) mRNA in cells treated with 3 or 100 μM FAC or 100 μM DFO for 24 h. Changes in Egr1 transcripts over 24 h after DFO addition (c). Changes in Egr1 mRNA with DFO at 0, 10, 50, 100 μM for 24 h (d). Egr1 mRNA in presence or absence of actinomycin d (2.5 μg/ml) for 1 h before addition of 3 or 100 μM FAC or 100 μM DFO (e). Relative luciferase activity of Egr1-luc from −604 to +160 for cells incubated with 3 or 100 μM FAC or 100 μM DFO (f). Except Fig. 1c, all the treatments were done for 24 h. Data are expressed as degree of induction over control and are mean ± SE. Student’s t test was performed between the groups. *p < 0.05, **p < 0.01, ***p < 0.001. All experiments were performed at least three independent times