Fig. 2

Effects of DFO on phosphorylation of ERK and Elk-1. Phosphorylated protein levels of ERK (p-ERK) and JNK (p-JNK) (a) and Elk (p-Elk) (e) in cells treated with 3 or 100 μM FAC or 100 μM DFO. Time course changes in p-ERK, ERK, p-JNK and TfR1 in cells incubated with 100 μM DFO (b). Egr1 mRNA from cells pretreated with or without PD98059 in the presence of 3 or 100 μM FAC or 100 μM DFO (c). Cells were transfected with 500 ng of p5X SRE reporter and 100 ng of pRLSV40. Next day transfection medium was changed with complete medium. The cells were stimulated with 3 or 100 μM FAC or 100 μM DFO for 24 h. Afterward, the cells were lysed and measured for luciferase activity (d). Cells were transfected with 250 ng of pEgr1-luc, 50 ng of pRLSV40 and 100 ng of pElk-1 S383A or empty vector. In following day, the cells were treated with vehicle or 100 μM DFO for 24 h. Relative luciferase activities in lysed cells were measured (f). Data are expressed as degree of induction over control and are mean ± SE. Student’s t test was performed between the groups. *p < 0.05, **p < 0.01, ***p < 0.001. All experiments were performed at least three independent times