Fig. 2

Curcumin-induced increase in the SMN2_E7+ variant is inhibited by trichostatin A. a RT-PCR analysis of the SMN2 variants (E7+ and E7−) in the cytoplasm of the BJ301J fibroblast cells upon 2 h of pretreatment with TSA or OA followed by 24-h addition of curcumin. GAPDH RNA loading control. b A bar graph represents the molar percentages of exon 7-containing SMN2 transcript (SMN2_E7+ mol%) are calculated as the molar intensity of the 364-bp product (SMN2_E7+) relative to the total of the 364- and 310-bp (SMN2_E7−) products (mean ± SD, n = 3) in each lane of the gel in a. *p < 0.05, **p < 0.01, comparison between the indicated samples