Fig. 3
From: Multiple effects of curcumin on promoting expression of the exon 7-containing SMN2 transcript
SRSF1 is specifically increased by curcumin. a Representative western blots of total proteins of splicing factors involved in the regulation of the SMN2 exon 7 alternative splicing upon 24-h treatment of BJ301J cells with DMSO or curcumin. β-ACTIN protein-loading control. b Immunostaining of splicing factors (green) in the BJ301J cells upon 24-h treatment with DMSO or curcumin. Nuclei are stained with DAPI (blue). c A bar graph of the transcript levels by RT-PCR analysis of the splicing factors (mean ± SD, n = 3) upon 24 h of treatment with DMSO or curcumin. Each transcript was normalized to that of GAPDH and then to that of the DMSO-treated sample, which is set as 100 %. **p < 0.01, ***p < 0.001, compared to DMSO-treated samples