Fig. 3

Real-time RT-PCR analysis of SLC30A mRNAs isolated from cells of Mother 1, Mother 2 and corresponding controls. a Comparison of SLC30A1, SLC30A2, SLC30A3, SLC30A4, SLC30A5, SLC30A6, SLC30A7, SLC30A8, SLC30A9 and SLC30A10 relative mRNA expression levels between lymphoblasts of Mother 1 and corresponding controls. A significant reduction in SLC30A5 and SLC30A6 transcript levels was detected in Mother 1 lymphoblasts compared to controls (p < 0.05, t test). No differences were observed in relative mRNA expression levels of SLC30A1, SLC30A3, SLC30A7, SLC30A9 and SLC30A10 in lymphoblasts from Mother 1 and Mother 2 versus controls (p > 0.05, t test). There was no detectable expression of SLC30A2 or SLC30A8 in lymphoblasts. b Comparison of SLC30A1, SLC30A2, SLC30A3, SLC30A4, SLC30A5, SLC30A6, SLC30A7, SLC30A8, SLC30A9 and SLC30A10 relative mRNA expression levels between fibroblasts of Mother 1 and corresponding control. A significant reduction in SLC30A5 and SLC30A6 mRNA in Mother 1 fibroblasts was found compared to controls (p < 0.05, t test). There was no detectable expression of SLC30A2 or SLC30A8 in fibroblasts. c In human resting breast tissue, SLC30A5 and SLC30A6 expressions were detected and a 48-fold difference was observed between SLC30A5 and SLC30A6 transcript levels. Values represent mean of triplicates ± SEM, *p ≤ 0.05