Fasting enriches liver triacylglycerol with n-3 polyunsaturated fatty acids: implications for understanding the adipose–liver axis in serum docosahexaenoic acid regulation

Table 5 Relative percentage of fatty acids in liver TAG

Fatty acid	Ad libitum	Fasted
Liver TAG (% weight total fatty acids)
C 10:0	0.07 ± 0.04	0.05 ± 0.02
C 12:0	0.10 ± 0.08	0.02 ± 0.02
C 14:0	0.06 ± 0.03	0.05 ± 0.03
C 16:0	25.76 ± 4.65	22.63 ± 2.76
C 18:0	3.50 ± 0.66	1.49 ± 0.35*
C 20:0	0.12 ± 0.07	0.04 ± 0.01
C 22:0	0.05 ± 0.05	0.02 ± 0.02
C 24:0	0.04 ± 0.03	0.01 ± 0.01
Total SFAs	34.25 ± 5.79	27.54 ± 1.55
C 12:1	0.10 ± 0.07	0.05 ± 0.01
C 14:1	0.21 ± 0.18	0.16 ± 0.03
C 16:1	4.37 ± 2.37	4.12 ± 1.31
C 18:1n-7	4.46 ± 1.16	2.54 ± 0.58*
C 18:1n-9	44.04 ± 4.92	35.35 ± 3.41*
C 20:1n-9	0.75 ± 0.11	0.38 ± 0.04*
C 22:1n-9	0.30 ± 0.10	0.16 ± 0.07
C 24:1n-9	0.05 ± 0.03	0.04 ± 0.05
Total MUFAs	54.28 ± 6.68	42.79 ± 4.29*
C 18:2n-6	9.37 ± 5.11	24.15 ± 2.74*
C 18:3n-6	0.27 ± 0.06	0.78 ± 0.07*
C 20:2n-6	0.19 ± 0.09	0.19 ± 0.05
C 20:3n-6	0.25 ± 0.13	0.29 ± 0.08
C 20:4n-6	0.43 ± 0.27	1.14 ± 0.13*
C 22:2n-6	0.07 ± 0.05	0.01 ± 0.01
C 22:4n-6	0.11 ± 0.04	0.15 ± 0.03
C 22:5n-6	0.06 ± 0.04	0.12 ± 0.03*
Total n-6	10.75 ± 5.73	26.85 ± 2.71*
C 18:3n-3	0.32 ± 0.17	0.90 ± 0.15*
C 20:3n-3	0.03 ± 0.02	0.02 ± 0.01
C 20:5n-3	0.04 ± 0.02	0.29 ± 0.08*
C 22:5n-3	0.07 ± 0.01	0.26 ± 0.05*
C 22:6n-3	0.26 ± 0.10	1.35 ± 0.15*
Total n-3	0.72 ± 0.29	2.82 ± 0.31*
n-6/n-3	14.3 ± 2.54	9.60 ± 1.20*
Total (μg/mg tissue)	13.41 ± 3.42	44.53 ± 9.52*

Data are mean ± SD (n = 3–4)
Significant differences between ad libitum fed and fasted mice are denoted by * p < 0.05

ISSN: 1865-3499