Fig. 2

mRNA array plots and QPCR analysis. a log2 scatter plot of hypothalamus mRNA array data from Affymetrix array. Data were filtered using a P value <0.05 and 1.3-fold change. b Volcano plot showing P value versus log2 expression level for data from Affymetrix array. Lines indicate where significance cutoff values were made. c QPCR analysis of selected mRNAs that were differentially expressed in food-deprived versus ad lib mice. d mRNAs were selected from cell cycle GO categories, and their expression levels tested by QPCR using N = 5–6 new samples for each group. All mRNA levels are reported as relative to levels in ad lib fed animals and normalized to the housekeeping gene β-actin. e String v10 was used to generate a network using the proteins tested in 7A as input. The network (interactive network mode) was expanded by one level. Only the names of the proteins input are shown. *P ≤ 0.05; **P ≤ 0.01