Fig. 3

Expression of p21 protein in non-transfected and miR-106b mimic-transfected LT97 cells after 24 h (a) and 48 h (b) and Cyclin D2 protein expression in non-transfected and miR-135a mimic-transfected LT97 cells after 24-h (c) and 48-h (d) incubation with 2, 4, 10 mM butyrate and 3.3 µM TSA (trichostatin A). Results represent fold changes (fc) on the basis of a medium control (MC = 1; dashed line). Significant differences ***P ≤ 0.001, **P ≤ 0.01; *P ≤ 0.05 to MC; ^# P ≤ 0.05 between transfected and non-transfected cells; ^a,b,c P ≤ 0.05 between concentrations (represented by different letters); Student’s t test (mean + SD, n = 3 independent experiments)