Table 2 Sequences of primers for hepcidin, DMT1, Fpn1, Dcytb, IRP1, IRP2, and β-actin
From: Effects of ferrous carbamoyl glycine on iron state and absorption in an iron-deficient rat model
Genes | Primer | 5′–3′ | Products (bp) |
|---|---|---|---|
Hepcidin | Forward | GCTGCCTGTCTCCTGCTTCT | 160 |
Reverse | TGGTGTCTCGCTTCCTTCG | ||
DMT1 | Forward | CTACTTGGGTTGGCAGTGTTTG | 102 |
Reverse | CCGCTGGTATCTTCGCTCA | ||
Fpn1 | Forward | TCGTAGCAGGAGAAAACAGG | 145 |
Reverse | GAAATGAAACCACAGCCAAT | ||
Dcytb | Forward | TCTGGACTCCTCCTCTTTGG | 107 |
Reverse | TCTGGTGGGAATGAATGGT | ||
IRP1 | Forward | TGGGGTGTAGGTGGTATTGA | 155 |
Reverse | TGTCGGAGGTGCTTGGTA | ||
IRP2 | Forward | AAGCCGAAACTCAGGAACA | 193 |
Reverse | AGGAGGGATGACTGCCACA | ||
β-Actin | Forward | TCGTGCGTGACATTAAAGAG | 134 |
Reverse | ATTGCCGATAGTGATGACCT |