Moderately decreased maternal dietary energy intake during pregnancy reduces fetal skeletal muscle mitochondrial biogenesis in the pigs

Table 2 Primer sequences of the target and reference genes

Gene	Primer sequence (5′–3′)	Product size (bp)	GeneBank no.
PPARGC1A	F: CCCGAAACAGTAGCAGAGACAAG R: CTGGGGTCAGAGGAAGAGATAAAG	111	NM_213963
SIRT1	F: TGACTGTGAAGCTGTACGAGGAG R: TGGCTCTATGAAACTGCTCTGG	143	EU030283.2
NRF1	F: GCCAGTGAGATGAAGAGAAACG R: CTACAGCAGGGACCAAAGTTCAC	166	AK237171.1
TFAM	F: GGTCCATCACAGGTAAAGCTGAA R: ATAAGATCGTTTCGCCCAACTTC	167	NM_001130211
ATP5B	F: CATGAAGCAGGTGGCAGGTA	127	NM_001185142
	R: CAGACGAACACCACGACTCA
POLG	F: CTTTGAGGTTTTCCAGCAGCAG	119	XM_005653521.1
	R: GCTCCCAGTTTTGGTTGACAG
SSBP1	F: CTTTGAGGTAGTGCTGTGTCG	142	XM_005673118.1
	R: CTCACCCCTGACGATGAAGAC
COX4	F: CCAAGTGGGACTACGACAAGAAC	131	Liu et al. [22]
	R: CCTGCTCGTTTATTAGCACTGG
CYCS	F: TAGAAAAGGGAGGCAAACACAAG	154	Liu et al. [22]
	R: GGATTCTCCAGGTACTCCATCAG
CS	F: CCTTTCAGACCCCTACTTGTCCT	127	Liu et al. [22]
	R: CACATCTTTGCCGACTTCCTTC
CuZn-SOD	F: AACATGGTGGGCCAAAGGAT	136	NM_001190422.1
	R: CGGCCAATGATGGAATGGTC
ACTB	F: TCTGGCACCACACCTTCT	114	DQ178122
	R: TGATCTGGGTCATCTTCTCAC
MT-CYB	F: ATGAAACATTGGAGTAGTCCTACTATTTACC R: CTACGAGGTCTGTTCCGATATAAGG	149	NC_000845.1
18S rRNA	F: GGTAGTGACGAAAAATAACAATACAGGAC	141	NC_010448.3
	R: ATACGCTATTGGAGCTGGAATTACC

ACTB β-actin, COX cytochrome c oxidase, CS citrate synthase, CuZn-SOD copper/zinc superoxide peroxidase, MT-CYB mitochondrially encoded cytochrome b, CYCS cytochrome c, somatic, ATP5B β subunit of mitochondrial H⁺-ATP synthase, NRF1 nuclear respiratory factor 1, PPARGC1A peroxisome proliferator-activated receptor gamma coactivator 1α, POLG gamma DNA polymerase, SIRT1 sirtuin 1, SSBP1 mitochondrial single-strand DNA-binding protein, TFAM mitochondrial transcription factor A

ISSN: 1865-3499