Fig. 1

Mild dehydration was induced in infant mice. To observe the effects of prolonged dehydration on development, an experiment was performed from infancy to the juvenile period. The restriction group had limited access to the water bottle for 15 min a day. The water restriction group had reduced daily water intake compared to the control group (a). Mild dehydration was generated, resulting in elevated plasma osmolality (b) and brain vasopressin mRNA level (c) in the dehydrated mice. The number of samples in each group is as follows: CON 1W (n = 4), RES 1W (n = 4), CON 4W (n = 3), and RES 4W (n = 4). Data are expressed as mean ± SEM. Student’s t test; **p < 0.01, ***p < 0.001 versus control group