Fig. 1

Relative levels of specific mRNA for Elovl-2, Elovl-5, and Elovl-6 in the liver of HiCHO and control groups. HiCHO high-carbohydrate (68%) diet-fed study group (n = 6), ST rats standard diet-fed controls (n = 6). Male Wistar rats weighing 151 ± 3 g were fed until achieved 250 g (21 days HiCHO vs. 25 days ST). The expression was determined by RT-PCR. Each gene was amplified together with a housekeeping gene β-actin (Actb; internal control). PCR products were separated on 1.5% agarose gel, stained with ethidium bromide, and analyzed by densitometry using the Quantity One 4.2.1. software. Obtained data were calculated to the mean value of the 200-bp band marker first and then normalized to β-actin (in the same sample). Shapiro-Wilk test was used for normality assessing. Student’s t test for unpaired samples; *p < 0.05