From: Omega-3 fatty acid DHA modulates p53, survivin, and microRNA-16-1 expression in KRAS-mutant colorectal cancer stem-like cells
Primer
Sequence (5′-3′)
PCR conditions*
Amplicon size (bp)
Survivin-F
TCCACTGCCCCACTGAGAAC
62(40)
80
Survivin-R
TGGCTCCCAGCCTTCCA
GAPDH-F
GAGTCCACTGGCGTCTTCA
62(35)
140
GAPDH-R
TCTTGAGGCTGTTGTCATACTTC