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Table 1 List of oligonucleotides used in the present study

From: Omega-3 fatty acid DHA modulates p53, survivin, and microRNA-16-1 expression in KRAS-mutant colorectal cancer stem-like cells

Primer

Sequence (5′-3′)

PCR conditions*

Amplicon size (bp)

Survivin-F

TCCACTGCCCCACTGAGAAC

62(40)

80

Survivin-R

TGGCTCCCAGCCTTCCA

  

GAPDH-F

GAGTCCACTGGCGTCTTCA

62(35)

140

GAPDH-R

TCTTGAGGCTGTTGTCATACTTC

  
  1. *Annealing temperature in degrees celsius (number of cycles)