Investigation of the influence of high glucose on molecular and genetic responses: an in vitro study using a human intestine model

Table 1 Effect of high glucose on cell permeability

	Ctrl	+Glc	p value	Significance
TEER (ohm/cm²)	934 ± 32.35	809.5 ± 39.29	0.0707	NS
Normalized OCLN mRNA levels	1 ± 0.095	0.93 ± 0.10	0.6096	NS
Normalized ZO1 mRNA levels	1 ± 0.064	1.05 ± 0.14	0.7336	NS

Caco-2 cells were grown on collagen-coated inserts in 5.5 and 25 mM glucose containing DMEMs for 21 days. TEER value was measured before RNA isolation. Occludin (OCLN) and Zoolin1 (ZO1) mRNA levels were measured by RT-qPCR, and relative fold changes of gene expression levels were analyzed. Statistical significance was determined by Student’s t test. Values are expressed as the mean ± SD; n = 3 independent experiments with three technical replicates

ISSN: 1865-3499