Investigation of the influence of high glucose on molecular and genetic responses: an in vitro study using a human intestine model

Table 5 Confirmation of mRNA expression of genes from molecular pathways

Genes	Ctrl	+Glc	p value	Significance
Group1
SRP14	1.008 ± 0.046	1.419 ± 0.102	0.002	Yes
SRP54	1.038 ± 0.101	1.804 ± 0.113	0.0003	Yes
SRP72	1.095 ± 0.20	1.177 ± 0.101	0.7509	NS
Group 2
UBE2L6	1.002 ± 0.151	2.034 ± 0,185	0.0007	Yes
UBE2Q2	1.000 ± 0.136	1.578 ± 0.139	0.01	Yes
SKP1	1.005 ± 0.167	1.516 ± 0.151	0.03	Yes
Group 3
ALDOA	1.000 ± 0.087	0.6652 ± 0.042	0.006	Yes
ALDH2	1.000 ± 0.138	0.3593 ± 0.146	0.01	Yes
PFKP	1.000 ± 0.088	0.3360 ± 0.074	0.0002	Yes
PGAM1	1.000 ± 0,108	0.5777 ± 0.138	0.03	Yes
PGD	1.000 ± 0.101	0.4760 ± 0.066	0.002	Yes
PFKFB3	1.000 ± 0.179	0.2334 ± 0,082	0.003	Yes

The gene expression levels from molecular pathways were analyzed by RT-qPCR method. The relative changes of mRNA expression levels in the protein export (group1) and ubiquitin-mediated proteolysis (group2) pathways were indicated in the table. The mRNA expression of common genes from molecular pathways that were downregulated by high glucose treatment was shown as group 3 in the table. Statistical significance was determined by Student’s t test. Values are expressed as the mean ± SD; n = 3 independent experiments with three technical replicates

ISSN: 1865-3499