Fig. 3

Effect of palmitate on lipid droplet formation in pMECs. Cells were incubated for 24 h with different concentrations of palmitate (0 (control), 25, 50, 100, 200, 400, and 600 μM) and then stained with Oil Red O and visualized by light microscopy with × 400 magnification. a 0 (control); b 25 μM; c 50 μM; d 100 μM; e 200 μM; f 400 μM; g 600 μM. h Maximal lipid droplet diameter. In h, data are expressed as mean ± SEM (n = 5) and different letters indicate statistical significance between different concentrations of palmitate treatment groups (p < 0.05)