High nutrient intake during the early postnatal period accelerates skeletal muscle fiber growth and maturity in intrauterine growth-restricted pigs

Table 1 Primer sequences of target and reference genes

Gene	Primer sequence (5′–3′)	Product size (bp)	Genbank ID
IGF-1	F: GAACTGAAGAGCGTCCACCA	81	NM_214256.1
IGF-1	R: TGCTTGCTCTCCTTCACCAG	81	NM_214256.1
IGF-1R	F: ATGGATCACAAAGCCCTCGG	148	HQ322390.1
IGF-1R	R: CTGCCGCCACTACTACTACG	148	HQ322390.1
GHR	F: GCTGTATGGATCCAGGGCTC	144	NM_214254.2
GHR	R: TGCAGAGAGTTCATCCAGGC	144	NM_214254.2
Akt	F: TCCAGCTTGAGGTCCCGATA	132	NM_001159776.1
Akt	R: GCTCTTCTTCCACCTGTCCC	132	NM_001159776.1
mTOR	F: GGGGTTTGGATCAGGGTCTG	80	XM_003127584.4
mTOR	R: GACTCATCCGCCCCTACATG	80	XM_003127584.4
RPS6K	F: TTGAACTTCTCCAGCGTCCC	106	XM_003131671.3
RPS6K	R: GCCTCCCTACCTCACACAAG	106	XM_003131671.3
RPS6	F: TACTCAGTAGCAGGCGGACT	92	XM_005660083.1
RPS6	R: TACTCAGTAGCAGGCGGACT	92	XM_005660083.1
eIF4E	F: ATGGAAGTCACTGTGGCCTG	133	DQ826509.1
eIF4E	R: TCGTCCCACTAGCTCACAGA	133	DQ826509.1
eIF4EBP1	F: CACAGGTGAGTTCCGACACT	105	NM_001244225.1
eIF4EBP1	R: GACTACAGCACCACTCCCG	105	NM_001244225.1
MyHC I	F: AAGGGCTTGAACGAGGAGTAGA	130	AB053226
MyHC I	R: TTATTCTGCTTCCTCCAAAGGG	130	AB053226
MyHC IIa	F: GCTGAGCGAGCTGAAATCC	155	AB025260
MyHC IIa	R: ACTGAGACACCAGAGCTTCT	155	AB025260
MyHC IIb	F: ATGAAGAGGAACCACATTA	137	AB025261
MyHC IIb	R: TTATTGCCTCAGTAGCTTG	137	AB025261
MyHC IIx	F: AGAAGATCAACTGAGTGAACT	113	AB025262
MyHC IIx	R: AGAGCTGAGAAACTAACGTG	113	AB025262
18S rRNA	F: GACTCAACACGGGAAACCTCAC	146	AY265350.1
18S rRNA	R: ATCGCTCCACCAACTAAGAACG	146	AY265350.1

IGF-1 insulin-like growth factor 1, IGF-1R insulin-like growth factor 1 receptor, GHR growth hormone receptor, Akt protein kinase B, mTOR mammalian target of rapamycin, RPS6K ribosomal protein S6 kinase, RPS6 ribosomal protein S6, eIF4E eukaryotic translation initiation factor 4E, eIF4EBP1 eukaryotic translation initiation factor 4E binding protein 1, MyHC myosin heavy chain

ISSN: 1865-3499