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Table 4 Primer sequences

From: Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs

Target gene

Primer sequence (5′ to 3′)

Accession no.

Product size (bp)

FABP3

Forward: CGCCTGTTCTGTCGTCTC

NM_001099931.1

263

Reverse: TCTCATCAAACTCCACTCCC

GHR

Forward: TGCCCAGGTAAGCGACAT

NM_214254.2

272

Reverse: TGCCAGCAGTAGTGGTAAGG

HSPA1L

Forward: CCATGAATCCCCAGAACACT

NM_001123128.1

313

Reverse: ATGACGCCTGCATCCTTGGT

Lpin1

Forward: TTCTCACAACACTGACCCTCTT

EU164847

330

Reverse: GCCCTCCGACTCTAACCAC

MB

Forward: CCCGAGACCCTGGAGAAAT

NM_214236.1

457

Reverse: CAACACTCAGAAGCAAACCCTAC

MSTN

Forward: AATGAGAACAGCGAGCAA

AY448008.2

279

Reverse: TTCCGTCGTAGCGTGATA

PFKM

Forward: ACCGAGTGCTGGTTGTGC

NM_001044550.1

305

Reverse: GTTGTTGGAGACCGTGGC

PPARGC-1

Forward: AAAGCCAACCAAGATAACCC

NM_213963.1

242

Reverse: TCACCAAATAGCCGCAGAC

LPL

Forward: CGACTGGATGGCGGTTG

NM_214286

271

Reverse: ATGCCCTACTGGTTTCTGGAT

TNNT3

Forward: CCCTCATCGACAGCCACTT

NM_001001863.1

318

Reverse: GCACCTTCTTCTTCATCTCCC

TNNI1

Forward: TGAACGACCTCCAGAAAGC

NM_213912.3

345

Reverse: GGCGGACACTCAGGAATAA

DECR1

Forward: TAAAGCACAGAAAGGAGCAG

NM_001190232.2

217

Reverse: TCCAGTTGGGTCAAGACG

FASN

Forward: CCTCATCGGCGGTGTGGA

NM_004104

122

Reverse: TGAAGTCGAAGAAGAAGGAGAGC

  

PCK1

Forward: ACAGCCTGCCCCAGGC

NM_001123158

148

Reverse: TTCAGCCTCTTGATGAC

  

18S RNA

Forward: GAGCGAAAGCATTTGCCAAG

AF102857

101

Reverse: GGCATCGTTTATGGTCGGAAC

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Genes & Nutrition

ISSN: 1865-3499

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