Fig. 3
From: Estrogen biosynthesis in cultured skeletal muscle cells (L6) induced by amino acids
a, b Western blot of MVD proteins. a MVD-protein levels increased almost equally when confluent L6 cells were cultured in the presence of BCAA concentrations only (2.8 mM), compared to all amino acids at high (9 mM) and low amino acid concentrations (0.3 mM). * p < 0.01 vs BCAA and high AA; n = 3 per group. b MVD proteins appeared at expected 43 kDa as a single band. Equal amounts of total protein/sample were applied (30 μg) and confirmed by Ponceau S staining of membranes. Chemiluminescent signals were quantified in ChemiDoc imaging system and Quantity One software