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Table 1 Characteristics of bacterial sequences in different groups

From: Alteration of gut microbiota affects expression of adiponectin and resistin through modifying DNA methylation in high-fat diet-induced obese mice

Sequences

Diversity and richness

 

Valid

Normalization

OTU

Coverage (%)

Ace

Chao

Shannon

Simpson

NC

381,500

300,670

3618

99.77 ± 0.00

404.73 ± 17.71

406.27 ± 19.92

4.09 ± 0.14

0.04 ± 0.01

NC-AB

385,442

300,670

654

99.89 ± 0.00

143.14 ± 37.31*

108.87 ± 22.21*

2.00 ± 0.14*

0.19 ± 0.03*

DIO

372,402

300,670

3314

99.77 ± 0.00

380.34 ± 19.30*

379.55 ± 23.48*

4.26 ± 0.15*

0.03 ± 0.01*

DIO-AB

365,196

300,670

474

99.91 ± 0.00

120.19 ± 48.38*#

87.36 ± 30.22*#

1.57 ± 0.09*#

0.24 ± 0.03*#

  1. Three to 4-week-old C57BL/6J male mice were fed a high-fat diet to induce obesity (DIO group) for 16 weeks, with a normal-fat diet as control (NC group). Meanwhile, ampicillin (1 g/L) and neomycin (0.5 g/L) were delivered via drinking water to mice fed the high-fat diet (DIO-AB group) and the normal-fat diet (NC-AB group). Fecal microbiota was analyzed by 16S rRNA high-throughput sequencing. The number of OTUs, coverage percentages, richness estimators (ACE and Chao), and diversity indices (Shannon and Simpson) were calculated at 3% distance. n = 10 in each group. Data are means ± SD
  2. *Compared to the NC group, P < 0.05
  3. #Compared to the DIO group, P < 0.05
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Genes & Nutrition

ISSN: 1865-3499

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