Study | Study design | Sample size | Populationa | Intervention duration | Intervention description | RNA extraction | Microarray | Weight changea | Transcript responseb, c | Pathway responseb, d | ||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Effect of intervention | HR vs LR | Effect of intervention | HR vs LR | |||||||||
Crujeiras et al. 2008 [21] | Cohort | 9 | 44.8 ± 5.2 year, BMI: 44.8 (5.2) kg/m2, 100% male, 100% Caucasian | 8 weeks | 35% energy restriction, 55% carbohydrates, 30% lipids, 15% protein | Trizol | Agilent Human 1A Oligo V2 | − 8.9 ± 1.5 % | 158 increased 227 decreasede | NA | NA | 7e |
Harvie et al. 2016 [22] | Cohort | 20 (HR = 7) | 40.3 ± 3.2 years, BMI: 28.3 (3.1) kg/m2, 100% female, 96% Caucasian | 4–5 weeks (1 full menstrual cycle) | Overall 25% energy restriction with 2 consecutive days 65% energy restriction, 5 days no restriction, restriction days—100 g carbohydrates and 50 g protein, non-restricted days—45% low-GI carbohydrates, 30% lipids, 25% protein | RNeasy plus kit | Affymetrix U133 plus2 | − 3.3 kg | 0 | 0 | 8 | 20 |
Cohort | 13 | 39 ± 9 years, BMI: 42.5 (7.0) kg/m2, 100% female, ethnicity NA | 6 months | Roux-en-Y gastric bypass surgery | Phenol/chlorform | Illumina HumanHT-12 V4 | − 28.8 kg | 7 increased 21 decreased | NA | 13 | NA | |
Rendo-Urteaga et al. 2015 [24] | Cohort | 12 (HR = 6) | 13.17 ± 0.98f years, BMI-SDS: HR: 3.10 (0.54), LR 4.08 (0.43)f, 100% male, ethnicity NA | 10 weeks | Between 1300 and 9204 kcal/day calculated based on energy requirements and severity of obesity | Trizol | Affymetrix Human 1.1 ST | HR: − 4.47 kg (0.23)f LR: + 0.75 kg | 372 increased 456 decreased | 0 increased in HR 23 decreased in HR | 53 | 21 |
Samblas et al. 2018 [25] | Randomised control trialg | Discovery = 24, validation = 37 | 48.4 ± 3.5 years, BMI: discovery: HR: 34.9 (3.0) kg/m2, LR: 37.0 (2.1) kg/m2, validation: HR: 33.8 (3.6) kg/m2 LR: 36.1 (5.0) kg/m2, 54% male, ethnicity NA | 6 months | 30% energy restriction, 40% carbohydrates, 30% lipids, 30% protein | Trizol | Illumina HumanHT-12 V4 | Discovery: HR: − 10.5% ± 1.8%, LR: − 5.9% ± 1.7% Validation: HR: − 9.5% ± 2.0%, LR: − 5.7% ± 2.1% | NA | 20 increased in HR 136 decreased in HRe | NA | NA |
van Bussel et al. 2019 [26] | Randomised Control Trial | 40 | 60 (50–65)h years, BMI: 29.2 (3.0) kg/m2, 52% male, 100% Caucasian | 12 weeks | 20% energy restriction, 50% carbohydrates, 35% lipids, 15% protein | Trizol | Affymetrix Human 1.1 ST | − 5.6 ± 2.9 kg | 0 | NA | 15 | NA |