Evaluation of candidate reference genes for quantitative real-time PCR analysis in a male rat model of dietary iron deficiency

Table 1 BestKeeper descriptive statistics and ranking of reference genes in pair-fed iron-replete animals in all tissues

	*Hprt*	*Rps29*	*Rplp0*	*Tbp*	*Rpl27*	*Rpl22*	*Gapdh*	*Actb*	*Ppia*	*Rpl19*
Geo mean [CP]	22.68	17.72	19.08	24.32	18.3	17.89	17.92	17.13	19.89	22.72
CV	0.02	0.06	0.06	0.05	0.06	0.06	0.07	0.07	0.06	0.06
Min [CP]	21.28	15.75	17.05	22.54	15.77	15.87	16.13	14.85	17.79	19.52
Max [CP]	24.66	20.73	22.36	26.45	21.15	20.38	20.65	19.9	25.65	25.29
Std dev [± CP]	0.51	1.06	1.08	1.11	1.13	1.14	1.17	1.22	1.24	1.37
Min [x-fold]	− 2.64	− 3.92	− 4.08	− 3.45	− 5.78	− 4.04	− 3.46	− 4.85	− 4.27	− 9.18
Max [x-fold]	3.94	8.05	9.71	4.37	7.21	5.64	6.63	6.85	54.44	5.92
Std dev [± x-fold]	1.42	2.08	2.11	2.16	2.19	2.2	2.25	2.33	2.37	2.59
Ranking	1	2	3	4	5	6	7	8	9	10

Geometric mean (CP), coefficient of variance (CV), and standard deviation (± CP) of the Cq values for putative reference genes. RG are ordered from left to right according to their SD_{Cq value}. Reference genes with a SD_Cq value < 1.0 are considered to be an appropriate reference gene when assessing gene expression in the pair-fed animals. To determine the under- and over-expression of a reference gene relative to the gene’s geometric mean (x-fold), the min, max, and standard deviation are calculated by the BestKeeper software

ISSN: 1865-3499