Evaluation of candidate reference genes for quantitative real-time PCR analysis in a male rat model of dietary iron deficiency

Table 2 BestKeeper descriptive statistics and ranking of reference genes in iron-deficient animals in all tissues

	*Hprt*	*Rps29*	*Tbp*	*Rpl19*	*Rplp0*	*Ppia*	*Rpl27*	*Rpl22*	*Actb*	*Gapdh*
Geo mean [CP]	22.57	17.48	24.16	17.44	19.2	17.6	18.8	17.99	16.64	22.47
CV	0.03	0.05	0.04	0.05	0.05	0.06	0.06	0.06	0.07	0.07
Min [CP]	21.13	15.55	21.67	15.45	17.26	15.48	16.62	15.68	14.49	19.18
Max [CP]	24.39	18.96	25.7	18.51	20.59	19.05	20.78	19.59	18.16	25.85
Std dev [± CP]	0.64	0.89	0.91	0.93	0.94	1.01	1.06	1.08	1.14	1.48
Min [x-fold]	− 2.7	− 3.81	− 5.6	− 3.98	− 3.83	− 4.35	− 4.53	− 4.95	− 4.42	− 9.75
Max [x-fold]	3.55	2.79	2.91	2.11	2.62	2.73	3.95	3.02	2.88	10.4
Std dev [± x-fold]	1.56	1.86	1.88	1.9	1.92	2.01	2.09	2.12	2.2	2.8
Ranking	1	2	3	4	5	6	7	8	9	10

Geometric mean (CP), coefficient of variance (CV), and standard deviation (± CP) of the Cq values for putative reference genes. RG are ordered from left to right according to their SD_{Cq value}. Reference genes with a SD_Cq value < 1.0 are considered to be an appropriate reference gene when assessing gene expression in the iron-deficient animals. To determine the under- and over-expression of a reference gene relative to the gene’s geometric mean (x-fold), the min, max, and standard deviation are calculated by the BestKeeper software

ISSN: 1865-3499