Fig. 5

Effects of lycopene, CD274 knockdown, and their combination on EMT pathway. A Western bolt was used to detect PD-L1 and downstream key molecules p-IGF-1R, p-Akt1 (Ser473), p-Akt1 (Thr308), p-c-Raf, p-MEK1, p-ERK, p-FOXO1A, p-FOXO3A, and p-GSK-3β (Ser9) expressions. According to manufacturer’s instructions, there were two bands of PD-L1, p-ERK and p-FOXO3A owing to post-translation modifications. B Important transcript factors NF-κBp65, STAT3, and c-Myc; EMT-transcription factors Slug, Snail, and ZEB-1; and EMT markers E-cadherin, N-cadherin, and Vimentin expressions were measured by western bolt. According to manufacturer’s instructions, there were two bands of c-Myc owing to different splicing. C Western bolt was performed to analyze 25 μM lycopene and SiRNA knockdown on PD-L1 and downstream key molecules p-IGF-1R, p-Akt1 (Ser473), p-Akt1 (Thr308), and p-ERK expressions in SCC25 cells. According to manufacturer’s instructions, there were two bands of PD-L1, p-ERK owing to post-translation modification. *P < 0.05 vs NC, #P < 0.05 vs CD274. NC means negative control. CD274 also known as PD-L1