Fig. 1

Total folates, cellular uptake and particianing of folate, mtDNA content, mitochondrial membrane potential, and basal respirationin HAP1 and ΔSHMT2 cells. Homozygous loss of SHMT2 and low-folate medium decrease folate accumulation and basal respiration and increase mitochondrial membrane potential in HAP1 cells. A SHMT2 protein levels, B total folate levels, C cellular uptake and partitioning of folate, D mtDNA content, E mitochondrial membrane potential, and F oxygen consumption rate in HAP1 cells and ΔSHMT2 cells. SHMT2 protein levels were normalized to GAPDH, and densitometry was performed using ImageJ. Two-way ANOVA with Tukey’s post hoc analysis was used to determine media by genotype interaction and main effects of media and genotype with a statistical significance at p < 0.05. Levels not connected by the same letter are significantly different. Data represent means ± SD values, n = 2–6 per group. GAPDH, glyceraldegyde-3 phosphate dehydrogenase; SHMT2, serine hydroxymethyltransferase 2