Fig. 5

Protein levels and pyruvate kinase activity inShmt2^+/+ and Shmt2^+/− MEF cells. Low-folate medium decreases protein levels, and activity of pyruvate kinase in MEF cells and reduced Shmt2 expression increases PGC1α protein levels. A PKM1, PKM2, and PGC1α protein levels and B pyruvate kinase activity in Shmt2^+/+ and Shmt2^+/− MEF cells. PKM1, PKM2, and PGC1α protein levels were normalized to GAPDH, and densitometry was performed using ImageJ. Two-way ANOVA with Tukey’s post hoc analysis was used to determine media by genotype interaction and main effects of media and genotype with a statistical significance at p < 0.05. Levels not connected by the same letter are significantly different. Data represent means ± SD values, n = 2–4 per group with 2 embryo cells lines represented in each group. GAPDH, glyceraldegyde-3 phosphate dehydrogenase; PGC1α, PPARγ coactivator-1α; PKM1 and PKM2, pyruvate kinase M1 and M2 isoforms