Fig. 2

The effect of HSI, exercise, and FOXO-RNAi on climbing endurance and rapid climbing ability in flies. A Time to fatigue in 1-week-old and FOXO-RNAi flies. B Time to fatigue in 3-week-old and FOXO-RNAi flies. C Time to fatigue in 5-week-old and FOXO-RNAi flies. D Time to fatigue in 7-week-old and FOXO-RNAi flies. E Time to fatigue changed with age in FOXO-RNAi flies. F Climbing index in 3 s at different ages. For climbing endurance, the sample size was 200–220 flies for each group. P values for climbing endurance curves were calculated by the log-rank test. For climbing index measurement, the sample size was about 150–170 flies for each group. The 1-way analysis of variance (ANOVA) with least significant difference (LSD) tests was used to identify differences among the groups. Data are represented as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ns means no significant differences. Male MHC-gal4 flies were crossed to the female FOXO-UAS-RNAi line. “P{KK108590} VIE-260B” and “P{KK108590}VIE-260B > MHC-gal4” were represented as “FOXO-normal-expression-1(FOXO-NE1)” and “FOXO-RNAi,” respectively