Fig. 3

Fisetin increases cellular ATP levels. HT22 cells were untreated (ct) or treated with 20 μM iodoacetic acid (+ IAA) to simulate ischemia for 2 h followed by 2 h in fresh medium alone in the absence (control) or presence (+ fisetin) of 10 μM fisetin. Cell lysates were prepared and total ATP levels were measured using a chemiluminescent assay. Adapted from [58]