Fig. 7
Fisetin enhances proteasome activity in HT22 cells. HT22 cells were plated at 3 × 105 cells/dish in 60 mm dishes and grown for 24 h. The cells were then left untreated or treated with the indicated concentrations of fisetin for 6 or 24 h. The cells were harvested and assayed for proteasome activity as described [55] using fluorescent substrates for the chymotrypsin-like (Suc-LLVY-AMC), caspase-like (Z-LLG-AMC) and trypsin-like (Z-ARR-AMC) activity of the proteasome. Results are presented as mean ± SD and are the average of four to five independent experiments