Expression of “brown-in-white” adipocyte biomarkers shows gender differences and the influence of early dietary exposure

Table 1 Nucleotide sequences of primers

Gene^a	Forward primer (5′–3′)	Reverse primer (5′–3′)	Amplicon size (bp)
AdipoQ	GCTCAGGATGCTACTGTTG	TCTCACCCTTAGGACCAAG	241
ATGL	TGTGGCCTCATTCCTCCTAC	AGCCCTGTTTGCACATCTCT	271
CD36	GTCCTGGCTGTGTTTGGA	GCTCAAAGATGGCTCCATTG	319
CIDEA	TCAGACCCTAAGAGACAACACA	CATTGAGACAGCCGAGGA	164
CPT1	GCAAACTGGACCGAGAAGAG	CCTTGAAGAAGCGACCTTTG	180
FASN	CGGCGAGTCTATGCCACTAT	ACACAGGGACCGAGTAAT	222
GDI	CCGCACAAGGCAAATACATC	GACTCTCTGAACCGTCATCAA	159
GLUT4	GGCATGCGTTTCCAGTATGT	GCCCCTCAGTCATTCTCATC	233
HOXC9	CGGCAGCAAGCACAAAGA	AGAAACTCCTTCTCCAGTTCCA	138
LEP	TTCACACACGCAGTCGGTAT	AGGTCTCGCAGGTTCTCCAG	186
LPL	TATGGCACAGTGGCTGAAAG	CTGACCAGCGGAAGTAGGAG	157
PPARγ2	GATCCTCCTGTTGACCCAGA	TCAAAGGAATGGGAGTGGTC	164
RETN	CTCCTCCTTTTCCTTTTCTTCC	TAGTGACGGTTGTGCCTTCT	205
SCD2	TTTCTCATCATCGCCAACAC	CTTTCCGCCCTTCTCTTTG	189
SHOX2	GCTGACGGAGGTGTCCCCTGA	CGAAAAGCCTCTCCAGCTCGTT	143
SREBP1c	CCCACCCCCTTACACACC	GCCTGCGGTCTTCATTGT	198
TBx15	GGATGAGACAGGTGGTCAGTT	CACAGGCACAGGTT	164
UCP2	GGTCGGAGATACCAGAGCAC	ATGAGGTTGGCTTTCAGGAG	174
XBP1	TCCGCAGCACTCAGACTATGT	ATGCCCAAAAGGATATCAGACTC	129

ATGL adipose triglyceride lipase, CD36 cluster of differentiation 36, CIDEA cell death-inducing DFFA-Like Effector A, CPT1 carnitine palmitoyltransferase, FASN fatty acid synthase, GDI guanosine diphosphate dissociation inhibitor, GLUT4 facilitated glucose transporter 4, HOXC9 homeobox C9, LEP leptin, LPL lipoprotein lipase, PPARγ2 peroxisome proliferator activated receptor gamma 2, RETN resistin, SCD2 stearoyl-Coenzyme A desaturase 2, SHOX2 short stature homeobox 2, SREBP1c sterol regulatory element binding transcription factor 1c, TBx15 T-box 15, UCP2 uncoupling protein 2, XBP X-box binding protein 1
^aAdipoq, adiponectin

ISSN: 1865-3499