Effect of genistein on cholesterol metabolism-related genes in a colon cancer cell line
© Springer-Verlag 2008
Published: 4 March 2008
The major soy-derived isoflavones such as genistein has been demonstrated to possess anticarcinogenic activity in animal model systems. The present study was designed to investigate the effects of isoflavone genistein exposure at concentrations ranging from 0.01 to 50 μM on the LDL receptor and HMG-CoA reductase gene expression in the estrogen receptor positive DLD-1 human colon cancer cell line. LDL receptor and HMG-CoA reductase gene expressions were evaluated by reverse transcription followed by real-time PCR. Genistein induced an increase of LDL receptor gene expression and later decrease of HMG-CoA reductase mRNA expression in DLD-1 cells. These findings provide direct evidence on the role of genistein in regulating LDL receptor and HMG-CoA reductase gene expression in colon cancer.
Soybeans contain about 40% protein, and depending on the processing procedure, the protein content can reach over 90% as in soy protein isolate (SPI) that is usually used in soy-based infant formulas . Most of the attention in soy studies has been focused on the proteins and their associated isoflavones (ISF). Epidemiological evidences suggest that soy consumption is linked to a lower incidence of chronic diseases including coronary heart disease, atherosclerosis, Type 2 diabetes, osteoporosis, and certain types of cancer such as prostate and breast cancer [31, 39]. In these connections, soy isoflavones have been reported to have a variety of biological activities including estrogenic , antioxidative , anti-osteoporotic  and anticarcinogenic  activities. Furthermore, recent clinical trials  and animal studies [4, 21, 33] also showed that dietary soy proteins or ISF reduce the risk factors for cardiovascular diseases, lowering triglyceride, total and low density lipoprotein (LDL) cholesterol levels and increasing the ratio of HDL/LDL cholesterol. However, the reported beneficial health effects were quite variable in different studies and the lack of understanding in the molecular mechanisms by which lipid metabolism is impacted, may contribute a major part to the discrepancies.
Soy proteins and ISF regulate lipid metabolism via multiple cellular pathways (fatty acid biosynthesis, cholesterol synthesis, catabolism and uptake) and through modulation of the key transcription factors including SREBP, PPAR, TR, RAR, and LXR . The same multiple cellular pathways are disregulated in colon cancer [10, 23, 34–36].
Cholesterol is an essential component of cell membrane and the main pathway through which proliferating cells gain cholesterol is de novo synthesis of endogenous cholesterol regulated by the activity of 3-hydroxy-methylglutaryl-coenzymeA (HMG-CoA) reductase. Alternatively, the cellular uptake of circulating LDL after binding to its receptor represents another mechanism of cholesterol acquisition. Several evidences have demonstrated that LDL receptor have a role in cell growth and tumorigenesis [8–10]. We, previously, showed that 63.3% of colon cancer patients did not present LDL receptors and that the absence of LDL receptor predicted shorter survival [8, 9]. Additionally, the absence of LDL receptor in cancers was associated with enhanced endogenous cholesterol synthesis . A loss of LDL receptor in tumors is expected to remove feedback inhibition of HMG-CoA reductase, stimulating endogenous mevalonate pathway . Increased synthesis mevalonate and mevalonate-derived isoprenoids supports increased cell proliferation through the activation of growth-regulatory proteins and oncoproteins and by promoting DNA synthesis . In agreement with these observations the inhibition of mevalonate synthesis and the up-regulation of LDL receptor, therefore may be an effective strategy to impair the growth of colon malignant cells.
Factors that up-regulate LDL receptor gene include estrogen and phytoestrogen in HepG2 cells and in rat liver [7, 13, 15]. Moreover the inhibition of mevalonate synthesis may be exerted by plant isoprenoid and genistein on experimental breast cancer . Genistein has been also shown to decrease cholesterol synthesis in cultured HepG2  and inhibits HMG-CoA reductase activity in MCF-7 human breast cancer cells . It has been suggested that the soy proteins enhance the expression of the LDL receptor in cultured human hepatoma cells , animals, and hypercholesterolemic Type 2 diabetic patients [24, 25]. A similar effect has been observed with a soy protein polypeptide in cultured HepG2 cells .
Previously, we have demonstrated an estrogenic regulation of cholesterol biosynthesis and cell growth in DLD-1 colon cancer cells. Estrogens induced an early increase of LDL receptor at both mRNA and protein level and later decrease of HMG-CoA reductase activity and protein expression .
Then, in the present study, we aimed to investigate whether genistein might affect gene expression of LDL receptor and HMG-CoA reductase in DLD-1 cells, a human colon cancer cell line expressing estrogen receptor β, but lacked estrogen receptor α . If genistein regulation of LDL receptor and HMG-CoA reductase gene expression via estrogen receptor β exists, the use of genistein might be a new approach for colon cancer management.
Cell culture condition
Human colon adenocarcinoma-derived DLD-1 cells were obtained from the Interlab Cell Line Collection (IST, Genoa, Italy). Cells were routinely cultured in RPMI 1640 without phenol red supplemented with 10% fetal bovine serum (FBS), 1% nonessential amino acids, 2 mM glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, in monolayer culture, and incubated at 37°C in a humidified atmosphere containing 5% CO2 in air. At confluence, the grown cells were harvested by means of trypsinization and serially subcultured with a 1:4 split ratio. All cell culture components were purchased from Sigma-Aldrich (Milan, Italy).
In the experiments investigating the effect of genistein under estrogen-depleted culture conditions, DLD-1 cells were seeded at a density of 2 × 105 cells/5 ml of phenol red-free RPMI 1640 containing 10% FBS in 60 mm tissue culture dishes (Corning Costar Co., Milan, Italy). After 24 h, to allow for attachment, the medium was removed, and RPMI 1640 containing 5% dextran-coated charcoal-treated FBS was added to cell line.
The cells were incubated for further 24 h, and then the medium was replaced by fresh culture medium containing 5% charcoal-stripped FBS with genistein at increasing concentrations (0.01, 1, 10 and 50 μM) dissolved in dimethyl sulfoxide (DMSO). The cells were allowed to grow for established time and then trypsinized. The cell pellet obtained after low-speed centrifugation was used for subsequent analyses.
Before proceeding with the experiments, estrogen levels were evaluated in the estrogen stripped medium in which DLD-1 cells were seeded by using a specific radioimmunoassay; estrogen levels were found below the detection limit.
Each experiment included an untreated control and a control with the equivalent concentration of DMSO as had been used for adding phytoestrogen. The solvent reached a concentration not higher than 0.3% in all experiments.
Triplicate cultures were set up for each phytoestrogen concentration and for control, and each experiment was repeated four times.
Cell viability, determined using the trypan blue exclusion test, always exceeded 90%.
LDL receptor and HMG-CoA reductase gene expression
DLD-1 cells were cultured with 0.01, 1, 10 and 50 μM of genistein for 30 min, 1, 2, 5 and 24 h in order to evaluate LDL receptor mRNA expression and for 24 and 48 h to analyze mRNA expression of HMG-CoA reductase. Very precocious times of exposure to genistein were tested because LDL-R gene is considered an early gene [5, 30], on the contrary longer times of exposure to genistein are required in order to detect an effect in HMG-CoAR gene expression [13, 30]. In parallel experiments, LDL-R mRNA levels were analysed in DLD-1 cells exposed for 5 h to genistein (0.01 μM) alone and genistein (0.01 μM) plus ICI 182,780 (0.1 μM).
Sequences of amplification primers
Real-time PCR was carried out in iCycler Thermal Cycler System apparatus (Bio-Rad) using the following parameters: one cycle of 95°C for 1 min and 30 s, followed by 45 cycles at 94°C for 10 s, 55°C for 10 s and 72°C for 30 s and a further melting curve step at 55–95°C with a heating rate of 0.5°C per cycle for 80 cycles. The PCR products were quantified by external calibration curves, one for each tested gene, obtained with serial dilutions of known copy number of molecules (102–107 molecules). All expression data were normalized by dividing the amount of target by the amount of β-actin used as internal control for each sample. The specificity of the PCR product of each tested gene was confirmed by gel electrophoresis.
Assessment of cell proliferation
After DLD-1 cells had been cultured for 24 h with different concentrations of genistein (0.01, 1, 10 and 50 μM), proliferative response was estimated by [3H]-thymidine incorporation and colorimetric MTT-test (Sigma-Aldrich) as previously reported [28, 29].
The significance of the differences between the control group and each experimental group was evaluated with one-way analysis of variance and the Dunnett’ post test. Differences were considered significant at a 5% probability level.
Genistein effects on LDL receptor gene expression in DLD-1 cells
0.6 ± 0.03
0.62 ± 0.01
0.65 ± 0.02
0.63 ± 0.05
0.5 ± 0.04
0.5 ± 0.02
0.6 ± 0.05
0.6 ± 0.05
1.2 ± 0.01*
0.9 ± 0.02*
0.4 ± 0.02
0.5 ± 0.04
0.9 ± 0.03
1.1 ± 0.02*
1.1 ± 0.02*
1.1 ± 0.06*
0.9 ± 0.02*
1.3 ± 0.07*
1.0 ± 0.02*
1.2 ± 0.06*
1.5 ± 0.08*
1.2 ± 0.06*
1.4 ± 0.06*
1.2 ± 0.05*
1.0 ± 0.08*
Genistein effects on HMG-CoA reductase gene expression in DLD-1 cells
1.04 ± 0.03
1.02 ± 0.01
1.03 ± 0.01
0.8 ± 0.01
1.02 ± 0.01
0.6 ± 0.02*
0.92 ± 0.04
0.5 ± 0.01*
0.8 ± 0.02
0.6 ± 0.05*
Exposure of the DLD-1 cell line to increasing concentrations of genistein (from 0.01 to 50 μM) under estrogen-depleted culture conditions showed an evident antiproliferative action, measured by 3H-thymidine incorporation and the colorimetric MTT method.
As with 3H-thymidine incorporation, the formazan-generated MTT decreased in dose-dependent manner, from 0.01 to 50 μM of genistein concentrations after 24 h of exposure (data not shown).
Factors that up-regulate LDL receptor expression in normal and neoplastic cells include multiple growth factors, many hormones (such as insulin, glucagons, growth hormone) and steroid hormones, such as estrogens. Some authors have demonstrated, both in vivo and in vitro, a stimulation of LDL receptor mRNA and protein expression in hepatoma cell line HepG2 and in rat liver by a natural estrogen, 17β-estradiol, suggesting an estrogen regulation of hepatic LDL receptor expression [13, 15]. Recently, it has been showed that diets containing soy protein enhance the clearance of apoB-containing lipoproteins via LDL receptors expressed on the hepatocyte plasma membrane .
Dietary factors may regulate HMG-CoA reductase activity with implications for breast cancer development . Kojima et al. have demonstrated that genistein itself and/or its metabolites may suppress hepatic lipid synthesis .
In this report, we show that LDL receptor and HMG-CoA reductase gene expression are affected by genistein in DLD-1 cell line. The phytoestrogen genistein is present in relatively high amounts in soybeans and it is able to induce LDL receptor gene expression and down-regulate HMG-CoA reductase gene in DLD-1 cells via estrogen receptor β. The effect of genistein treatment on LDL receptor gene expression was dose-dependent after 2 h. A good time-dependency was present at 1 μM of genistein.
In addition, genistein, exerted an evident antiproliferative action on DLD-1 cells in a dose-dependent manner.
These findings provide direct evidence of the relationship between phytoestrogens and cholesterol pathway, representing a promising basis for the comprehension of the mechanism of estrogen and phytoestrogens action in gastrointestinal tract. We have already showed that estrogens modulate the colon cancer proliferation by the up regulation of LDL receptor and the down regulation of HMG-CoA reductase activity , suggesting that the cholesterol metabolism could be considered an additional target for the estrogenic antiproliferative properties in colon cancer.
The estrogen-gene regulated transcription utilizes different pathways depending on the cellular contex. It is known that at hepatic level the induction of LDL receptor gene by estrogen is mediated by non transcriptional factors, but if a similar mechanism exists in colon cells is not currently known. Moreover, genistein is also a tirosyne kinase inhibitor and therefore can affect the cell signal transduction pathway through this way. Further studies are required to identify this molecular mechanism.
HMG-CoA reductase is the key enzyme of mevalonate biosynthesis . A reduction of the HMG-CoA reductase expression by genistein may decrease the mevalonate pool, thus limiting protein isoprenylation, which involves the post-translational covalent attachment of a lipophilic farnesyl or geranylgeranyl isoprenoid group to several proteins . Many prenylated proteins, like Ras, lamin B, and Rho regulate cell growth and/or transformation. The up-regulation of LDL receptor by genistein make to an increased cholesterol intracellular content, modulating HMG-CoA reductase gene expression and consequently controlling cell proliferation and transformation.
Our findings support a variety of studies that have linked the soy-derived isoflavones, such as genistein, with anti-tumorigenic activity [6, 12]. Genistein has been demonstrated to reduce proliferation and induce G2/M phase arrest and apoptotic death in colon cancer HT-29 cells . Genistein increased expression of Bax and p21WAF1 and slightly decreased Bcl-2 level and resulted to inhibit the viability of human colon cancer HT-29 cells via induction of apoptosis. In a previous study, we showed that genistein can affect growth of DLD-1 cells by both decreasing polyamine biosynthesis and inducing apoptosis . It is known that high polyamine levels are associated with fast proliferating cells  and that polyamine levels in colon cancer are significantly increased compared to normal and preneoplastic tissue . Therefore, the reduction of polyamine levels represents an antiproliferative mechanism, offered by genistein, against colon cancer growth. Genistein produces suggestive effects for estrogenicity given that it can induce estrogen-responsive gene products, as LDL receptor. This estrogen agonistic activity of genistein may account for the beneficial effects of this compound against colon cancer development. A central role of genistein in prevention of colon tumor and in reduction of colon tumor growth might be considered.
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