Coffee intake mitigated inflammation and obesity-induced insulin resistance in skeletal muscle of high-fat diet-induced obese mice
© Springer-Verlag Berlin Heidelberg 2014
Received: 17 November 2013
Accepted: 17 February 2014
Published: 6 March 2014
Epidemiologic findings offer the promise that coffee or its many constituents may be useful as a dietary intervention in type 2 diabetes (T2D) prevention. We aimed to elucidate the molecular mechanisms involved in the ameliorative effects of caffeinated coffee (CC), decaffeinated coffee (DC) and unroasted caffeinated green coffee (GC) on skeletal muscle gene expression profiles and their relationships in an obesity animal model. Eight-week-old male C57BL6 mice were raised for 9 weeks ad libitum on a normal diet, a high-fat diet, or high-fat diet containing 2 % freeze-dried CC, or DC, or GC. Total RNA and protein were extracted from skeletal muscle and subjected to microarray (Mouse Genome 430 2.0, Affymetrix) and western blotting analyses, respectively. Coffee intake mitigated the insulin resistance by decreasing plasma glucose levels during an insulin tolerance test and by increasing tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1), p85/IRS-1 complex and pAkt/PKB (protein kinase B). In addition, coffee intake down-regulated the anti-inflammatory genes activating transcription factor 3, FBJ osteosarcoma oncogene, heat shock protein 1A, heat shock protein 1B and synuclein, gamma and the inflammation-associated insulin signaling genes stearoyl-coenzyme A desaturase 1 and secreted phosphoprotein 1. These results provide scientific insight on the probable positive effects of coffee intake on impaired insulin signaling, inflammation and obesity, thereby providing a new perspective on the prevention of obesity and T2D.
Coffee consumption is a trend that presumably originated in northeast Africa, spread across the Middle East in the fifteenth century and then to Europe (Gray 1998), and now, approximately 2.25 billion cups of coffee are consumed across the globe every day (Stefano 2002). Epidemiologic findings offer the promise that coffee, or one or more of its many constituents, may prove to be useful as a novel approach for type 2 diabetes (T2D) prevention (Hu et al. 2006; Paynter et al. 2006). The number of people estimated to develop T2D is rapidly increasing, and this is strongly associated with the changes in dietary habits. As such, it is of considerable importance to identify possible dietary interventions for the primary prevention of T2D.
Coffee is a complex mixture of hundreds of chemicals that occur naturally or are formed during the roasting process. Caffeic acid (CA) occurs in food mainly as esters known as chlorogenic acids (CGAs) (Rice-Evans et al. 1996). Roasting markedly affects the composition of coffee, and compounds with antioxidant properties (mainly CGAs) are lost during the roasting of coffee beans (Abebe and Kebba 2013). In a db/db diabetic mouse model, CA also exhibited anti-diabetic properties by markedly increasing glucokinase activity and simultaneously lowering glucose-6-phosphatase and phosphoenolpyruvate carboxykinase activities in the liver (Jung 2006). CGA has been reported to modulate the glucose-6-phosphatase involved in glucose metabolism and to reduce the risk of cardiovascular disease by decreasing the oxidation of low-density lipoprotein cholesterol and total cholesterol (TC) (Nardini et al. 1995). In diet-induced obese mice, CGAs also altered the plasma adipokine level and body fat distribution and down-regulated fatty acid and cholesterol biosynthesis, but they up-regulated fatty acid oxidation and hepatic peroxisome proliferator-activated receptor alpha expression (Cho et al. 2010).
In addition to the constituents of coffee, coffee itself has been reported to down-regulate the expression of inflammatory cytokines [i.e., monocyte chemotactic protein-1, interleukin (IL)-1β, IL-6, and leptin] in adipose tissue and liver (Matsuda et al. 2011), and to increase the insulin-induced serine phosphorylation of Akt/PKB (protein kinase B) in the liver and skeletal muscle of diabetic mice (Kobayashi et al. 2012). As highlighted above, most studies have focused on the effects of coffee intake on the liver and adipose tissue. The influence of coffee intake on the expression of inflammatory cytokines and the molecular mechanisms underlying the improved insulin sensitivity in skeletal muscle are relatively unknown, despite the fact that skeletal muscle is a fundamental player in glucose metabolism and homeostasis in humans, accounting for nearly 40 % of body mass (DeFronzo et al. 1992).
In recent years, applications of microarray technology, a robust tool in the global evaluation of gene expression profiles, have provided vital information regarding the use of nutraceuticals for the prevention and management of several human diseases (Jia et al. 2013a, b). In the present study, we used DNA microarray and western blotting analysis to investigate the influence of the intake of different types of coffee—caffeinated coffee (CC), decaffeinated coffee (DC), and unroasted caffeinated green coffee (GC)—on skeletal muscle gene expression profiles and their relationships in a high-fat diet-induced obesity animal model. We aimed to elucidate the molecular mechanisms involved in the ameliorative effects of coffee intake on impaired insulin signaling, inflammation and obesity, thereby providing new perspectives and scientific evidence to support the effectiveness of coffee intake in controlling obesity and T2D.
Materials and methods
Animals and dietary treatment
Seven-week-old male C57BL6J mice obtained from Charles River Japan (Tokyo) were housed individually in animal cages in a room with controlled temperature (23 ± 2 °C), humidity (50 ± 10 %), and lighting (lights on from 0800 to 2000). After 7 days of acclimatization, the mice were divided into five groups with equal mean body weights. The normal diet group was fed D12450B (ND; 10 % kcal fat), the high-fat diet group was fed D12492 (HF; 60 % kcal fat), and the three coffee groups were fed the high-fat diet containing 2 % CC (HFCC), or DC (HFDC), or GC (HFGC), respectively. Freeze-dried coffee (Takahashi et al. 2014) provided by Nestle Japan Ltd. (Tokyo) were mixed into the diets by Research Diets Inc. (New Brunswick, NJ, USA) (Supplementary Table 1). The mice received their respective diets and drinking water ad libitum for 9 weeks. Food intake and body weights were monitored throughout the experiment.
Blood collection and tissue harvesting
Prior to sacrifice, mice were starved for 16 h and were deeply anaesthetized with sodium pentobarbital, followed by bleeding from the carotid artery. The gastrocnemius muscle from the lower limb, the liver, and mesenteric and epididymal adipose tissues were excised and snap-frozen in liquid nitrogen and stored at −80 °C until further analysis. Plasma was obtained by centrifuging blood at 1,000g for 15 min at 4 °C. This study was approved by the Animal Care and Use Committee of The University of Tokyo, and the animals were treated in accordance with the committee’s guidelines.
Triglyceride (TG) and TC concentrations in plasma were measured using a cholesterol E test kit and a TG E test (Wako Pure Chemical Industries, Osaka, Japan) according to the manufacturer’s instructions. Total lipids in the liver were extracted according to the Folch method, after which the hepatic concentrations of TG and TC were measured by the tests mentioned above.
DNA microarray analysis
The total skeletal muscle RNA extraction and quality assessment were conducted as described (Jia et al. 2013a). Total RNA from each respective group were pooled (n = 4–5). The microarray analysis (Mouse Genome 430 2.0 Array Gene Chip, Affymetrix, Santa Clara, CA, USA) was carried as out as described (Jia et al. 2013a). Array images were analyzed with Microarray Suite version 5.0 (Affymetrix). After normalizing the differences in microarray intensities, we performed pairwise comparisons between the ND (as baseline) and HF values, and between the HF values (as the baseline) and those of the HFCC or HFDC or HFGC group (Supplementary Materials). Genes were considered to be differentially expressed if they were up- or down-regulated by 1.2-fold compared to the HF group. Genes were considered to be differentially expressed if they were up-or down-regulated by 1.2-fold compared to the HF group.
Real-time reverse transcription polymerase chain reaction
We performed a real-time reverse transcription polymerase chain reaction (RT-PCR) as described (Jia et al. 2013a) to detect the expression of genes related to inflammation, obesity, insulin resistance and T2D. Primers were designed with a Web application (PRIMER3), and their sequences are as shown in Supplementary Table 2. The relative amounts of mRNA were normalized to β-actin expression levels in each sample, and the data are presented as the fold change of normalized mRNA amounts of each sample compared to those of the ND group.
Quantification of Atf3 and Socs3 protein levels
Excised gastrocnemius muscle was minced and homogenized in 0.02 mol/L ice-cold phosphate-buffer saline (pH 7.1), after which homogenates were centrifuged for 15 min at 1,500g. We measured the protein levels of Atf3 and Socs3 in the supernatant using enzyme-linked immunosorbent assay (ELISA) kits following the manufacturer’s instructions (MyBioSource, San Diego, CA, USA). Muscle protein concentrations were measured using the Bradford assay. Data were then presented as the fold change of the normalized protein concentration comparing to HF group.
Insulin tolerance test
To further investigate the effects of coffee intake on insulin signaling in the skeletal muscle, we performed an animal experiment identical to that described above in the section “Animals and Dietary Treatment.” At week 8 of the experimental period, we performed an insulin tolerance test (ITT) with 4-h fasting. The solutions of bovine insulin (0.75 U/kg body weight) were injected i.p. Glucose levels were determined using Ascensia BREEZE (Bayer Medical Ltd., Leverkusen, Germany) in blood obtained from the tail vein at 0, 15, 30, 60, 120, and 150 min after the injection. We calculated the areas under the curve (AUC) as Glucose (AUCglucose).
In vivo insulin stimulation
At the end of the 9-week experimental period, in each group, the mice were divided again into saline (n = 4) and insulin (n = 5) groups. All mice were starved for 16 h and then re-fed for 1.5 h before being killed. The mice were deeply anaesthetized with sodium pentobarbital. As soon as the effect of the anesthesia was assured by the loss of pedal reflexes, the abdominal cavity was opened, and normal saline or 0.14 U/200 μL of bovine insulin (Sigma-Aldrich, Saint Louis, MO, USA) was injected into the cava vein. At 2 min after the insulin administration, skeletal muscles from the lower limb were excised and snap-frozen in liquid nitrogen. The frozen tissues were homogenized in a polytron homogenizer at 4 °C with 10 times the muscle weight of homogenizing buffer (50 mM HEPES–NaOH, pH 7.6, 10 mM sodium orthovanadate, 10 mM sodium pyrophosphate, 100 mM sodium fluoride, 2 mM phenylmethylsulfonyl fluoride, 100 KIU/mL aprotinin, 2 mM EDTA, and 2 % Triton X-100). The homogenate was centrifuged at 100,000g for 60 min at 4 °C, and the supernatants were collected for the western blotting analysis. The protein concentration was determined with a Bio-Rad Protein Assay kit (Bio-Rad, Gladesville, New South Wales, Australia).
Western blotting analysis of Ser473 and Akt
Twenty microgram protein lysates were suspended in Laemmli’s sample buffer, boiled for 5 min and used for Western blotting. Samples were subjected to SDS-PAGE on acrylamide gel, and the proteins in the gel were transferred onto PVDF membranes (Hybond P, GE Healthcare, Tokyo, Japan) and incubated overnight at 4 °C with anti-phospho Akt/PKB (Ser473) (1:1,000; Cell Signalling Technology, Danvers, MA, USA), or anti-Akt (4058S, 1:1,000; Cell Signalling Technology), and then washed with Tris-buffered saline containing 0.1 % Tween 20. The membranes were then incubated with 1:5,000 diluted horseradish peroxidase-conjugated secondary antibodies diluted in TBS-T at room temperature for 1 h, and washed again as described above. The bands of immunoreactive proteins were detected with an enhanced chemiluminescence kit (GE Healthcare, Buckinghamshire, UK) and quantified using an LAS-1000 plus (Fuji Film Co., Kanagawa, Japan).
Immunoprecipitation of IRS-1 and western blotting analysis of IRS-1, p85, and phosphotyrosine
For immunoprecipitation of insulin receptor substrate-1 (IRS-1), the extracted protein (4 mg) was incubated with anti-IRS-1 (4 μg) antibody at 4 °C overnight, followed by the addition of 30 μl protein G-Sepharose 4FF (50 % v/v) for 1.5 h. The immunocomplexes were washed three times with the homogenizing buffer at 4 °C, suspended in Laemmli’s sample buffer, and boiled for 5 min. Subsequent western blotting analysis using anti-IRS-1 (06-248, 1:1,000; Millpore, Billerica, MA, USA), or anti-phosphotyrosine (4G10, 1:1,000; Millpore), or anti-p85 (1:1,000; Millpore) was performed as described above.
Data are presented as mean values ± standard error (SE). The statistical analysis was conducted using a one-way analysis of variance, and significant differences in mean values were evaluated with Dunnett’s test at the level of p < 0.05 versus the HF group.
General characteristics of the diet groups
Body weight (g)
30.0 ± 0.9*
39.3 ± 1.3
36.0 ± 1.0*
35.2 ± 0.1**
35.8 ± 0.9*
Average daily food intake (g)
3.0 ± 0.0
3.0 ± 0.0
3.0 ± 0.1
2.9 ± 0.1
3.1 ± 0.0
Daily calorie intake (kcal/day)
11.3 ± 0.1**
15.8 ± 0.1
15.6 ± 0.5
15.3 ± 0.5
16.2 ± 0.1
Mesenteric fat (g)
0.3 ± 0.1*
0.7 ± 0.1
0.5 ± 0.1
0.4 ± 0.0*
0.5 ± 0.1
Epididymal fat (g)
0.7 ± 0.1**
2.3 ± 0.2
2.2 ± 0.3
2.2 ± 0.1
2.2 ± 0.1
Plasma insulin (ng/mL)
0.2 ± 0.0*
1.4 ± 0.4
1.0 ± 0.3
0.9 ± 0.2
1.3 ± 0.2
Plasma glucose (ng/mL)
141.2 ± 1.3
172.9 ± 2.9
168.9 ± 4.5
168.9 ± 4.0
173.6 ± 5.2
Plasma TC (mg/dL)
129.5 ± 20.0
151.8 ± 21.8
116.8 ± 13.0
153.9 ± 10.9
131.6 ± 3.2
Plasma TG (mg/dL)
59.0 ± 4.2*
73.0 ± 3.2
67.0 ± 4.3
66.0 ± 6.0
64.1 ± 4.4*
Liver weight (g)
0.9 ± 0.0**
1.1 ± 0.0
1.0 ± 0.0
1.0 ± 0.0
1.0 ± 0.0
Hepatic TC (mg/g)
3.9 ± 0.3
3.7 ± 0.1
3.1 ± 0.3
3.6 ± 0.2
3.1 ± 0.2
Hepatic TG (mg/g)
32.8 ± 3.5
37.9 ± 2.9
24.4 ± 2.3**
35.1 ± 2.1
26.5 ± 2.7*
Mice in the HFCC, HFDC, and HFGC groups gained significantly less weight as compared with mice in HF group. The DC significantly suppressed the increase in mesenteric fat weight, whereas the apparent suppression observed in CC and GC was not statistically significant. The epididymal fat weights tended to be lower in all coffee groups compared with the HF mice. Moreover, there was a significant decrease in hepatic TG levels in the HFCC and HFGC groups compared with the HF mice, whereas HFDC showed a tendency for decreased levels. There was no significant change in plasma TC in the HFDC mice, and the plasma and hepatic TC levels of all three coffee groups also tended to be lower than that of the HF group.
Comparison of gene expression profiles
We assessed the differences in muscular gene expression profiles between the HF and coffee groups in a DNA microarray analysis. Among the total genes analyzed, 285 genes in the HFCC mice, 247 genes in the HFDC mice, and 169 genes in the HFGC mice were differentially expressed compared with the HF mice. The list of commonly altered genes, 45 up-regulated and 17 down-regulated, is given in Supplementary Table 3.
Among the down-regulated genes, several are associated with modulating cellular stress and inflammatory response, including activating transcription factor 3 (Atf3, 0.5-fold in HFCC, 0.6-fold in HFDC, and HFGC), suppressor of cytokine signaling 3 (Socs3, 0.6-fold in HFCC, 0.5-fold in HFDC and 0.3-fold in HFGC), heat shock protein 1A (Hspa1a, 0.7-fold in HFCC and HFGC and 0.6-fold in HFDC), heat shock protein 1B (Hspa1b, 0.6-fold in HFCC, 0.8-fold in HFDC and 0.7-fold in HFGC). In addition, an enzyme involved in fatty acid biosynthesis, stearoyl-coenzyme A desaturase 1 (Scd1, 0.6-fold in HFCC, 0.7-fold in HFDC and 1.1-fold in HFGC), and genes involved in the PI3K/AKT signaling encoding pathway protein phosphatase 2, regulatory subunit B”, alpha (Ppp2r3a, 0.5-fold in HFCC and HFDC and 0.7-fold in HFGC) were also downregulated. Moreover, synuclein, gamma (breast cancer-specific protein 1, Sncg) which is an upstream regulator of Hspa1a, and Hspa1b was down-regulated 0.6-fold in HFCC, 0.5-fold in HFDC, and 0.9-fold in HFGC, and upstream regulator of Atf3, FBJ osteosarcoma oncogene (Fos) was also was decreased 0.4-fold in HFCC, 0.25-fold in HFDC, and 1.1-fold in HFGC.
In addition, genes that have been reported to be up-regulated in human diabetic subjects were observed to be down-regulated in the three coffee groups, including Ras-related associated with diabetes (Rrad, 0.5-fold in HFCC and HFGC, and onefold in HFDC) and secreted phosphoprotein 1 (Spp1, 0.6-fold in HFCC, 1.1-fold in HFDC and 0.9-fold in HFGC).
On the other hand, the several up-regulated genes include protein tyrosine phosphatase, non-receptor type 11 (Ptpn11, 1.4-fold in CC, 1.6-fold in DC, and onefold in GC), and phosphodiesterase 4D interacting protein (myomegalin) (Pde4dip, 1.5-fold in CC, 1.4-fold in DC and 1.2-fold in GC).
Validation of microarray results by RT-PCR
Atf3 and Socs3 protein concentration
In addition, the western blotting analyses revealed that the insulin administration markedly elevated the tyrosine phosphorylation of IRS-1, the levels of p85/IRS-1 complex, and the serine473 phosphorylation of Akt/PKB, most of which were suppressed in the HF group (Fig. 3). However, the tyrosine phosphorylation levels of IRS-1 tended to increase in all coffee groups compared with the HF group. The levels of the p85/IRS-1 complex were improved in the HFCC and HFGC mice, and the serine473 phosphorylated Akt/PKB levels also tended to higher in the HFCC group.
In the present study, we sought to assess the molecular mechanisms underlying the effects of coffee intake on impaired insulin signaling, inflammation and obesity, by conducting a comprehensive transcriptomic analysis integrated western blotting evaluation in mouse skeletal muscle, of which is the pivotal involvement in glucose metabolism and homeostasis in humans. Our results suggest that coffee intake may suppress the development of features leading to T2D.
Various dietary and lifestyle factors have been identified as contributing to the development of T2D, of which obesity has been labeled one of the most fundamental causes (Hu et al. 2001). Obesity is a systemic inflammatory response, and many inflammatory mediators exhibit expression patterns that interfere with insulin action (Pradhan 2003; Han et al. 2002; Barzilay et al. 2001; Pradhan et al. 2001). In the present study, the coffee intake by mice significantly suppressed the body weight gain and attenuated the increase in mesenteric fat weight and glucose levels during the ITT. As such, we may infer that obesity due to body weight gain and the increases in adipose tissue and insulin resistance brought about by a high-fat diet may be ameliorated by coffee intake; however, further conformational studies in humans are required.
The suppression of body weight gain and insulin resistance by coffee intake was reflected in the altered expression of several genes related to inflammation, insulin signaling, and obesity. Transcription factor Atf3 is induced by various stress signals such as high levels of glucose and fatty acids, endoplasmic reticulum stress, and reactive oxygen species (Zmuda et al. 2010) which are relevant to T2D. Atf3 and its transcription factor, Fos, have been found to be up-regulated in the skeletal muscle of insulin-resistant humans, where prolonged activation of proinflammatory pathways within insulin target cells which leads to obesity-related insulin resistance can be attributed to the activation of the JNK/activator protein 1 (AP1) signaling pathways composed of c-Jun and c-Fos proteins (Bossis et al. 2005). Moreover, a downstream target gene of Atf3, Socs3 which is a major negative regulator of both leptin and insulin signaling, (Jiang et al. 2004) impairs muscle insulin signaling and promotes systemic insulin resistance (Jiang et al. 2004). In the present study, the elevated expressions of Atf3, Fos and Socs3 in the mice fed an HF diet were reduced by coffee intake. In particular, CC and GC may improve insulin signaling in skeletal muscle, partially via the increased tyrosine phosphorylation of IRS-1 due to lowered Socs3 expression.
The Atf3 protein levels were also decreased in all three of the present coffee groups, with substantial decreases in the mice fed the HFDC and HFGC diets. CGA, a component of DC and GC, has been reported to exert anti-inflammatory effects via the attenuation of the activation of JNK/AP1 pathways (Shan et al. 2009). As CGA is abundant in DC and GC (Supplementary Table 1), we may infer that CGA may contribute to the reduction in Atf3 protein levels observed in the present HFDC and HFGC groups. Moreover, we can also deduce that the anti-inflammatory properties of CA which exists in high concentrations in CC and GC (Supplementary Table 1) may play a role in the reduced expression of Atf3, Fos and Socs3. This idea is also supported by the increased levels of p85/IRS-1 observed only in the caffeine-consuming mice.
The expression levels of Scd1 induced by Atf3 were also similarly increased in the HF group and significantly reduced in the HFCC group. Scd1 encodes for the microsomal enzyme stearoyl-CoA desaturase 1, which is involved in the conversion of saturated fatty acids (Peter et al. 2009). High SCD1 inducibility was associated with ATF3 expression, and this was positively correlated with intramyocellular lipids and insulin sensitivity (Peter et al. 2009). Another gene similar to Scd1 with an effect on inflammation-associated insulin signaling is Spp1. Spp1, a multifunctional protein, has an important role in a variety of inflammatory disorders, e.g., atherosclerosis, and in diabetic macro- and micro-vascular diseases and hepatic inflammation where the antibody-mediated neutralization of Spp1 in obese mice was reported to rapidly improve insulin sensitivity (Kiefer et al. 2010). In the present study, the CC significantly attenuated the increases in Scd1 and Spp1 expression induced by the HF diet, and the improvement in inflammation-associated insulin signaling was reflected by the increase in the tyrosine phosphorylation of IRS-1, p85/IRS-1 complex and Ser473 phosphorylated Akt/PKB levels and lower insulin levels.
Hspa1a and Hspa1b are two copies of genetically similar genes encoding heat shock protein 70 (Hsp70) (Smith et al. 2007) usually elevated during high levels of oxidative stress and glucose deprivation and have been implicated in immune-mediated type 1 diabetes, insulin resistance, and obesity-related T2D (Smith et al. 2007). The up-regulation of Hspa1a and Hspa1b in the present HF group compared to the ND controls indicates an inflammatory state brought about by the high-fat diet. The expression levels of these genes were significantly lower in the HFCC group and tended to be lower in HFGC group but were not changed in the HFDC group, suggesting that CA and CGA which exist in high concentrations in CC and GC might play an important role in reducing obesity-related inflammatory and oxidative insults (Chao et al. 2009; Jung 2006).
In addition, Sncg, a novel chaperone in the expression of Hsp70, encodes for the gamma-synuclein as a marker of peripheral and selects central nervous system neurons (Oort et al. 2008). Compromised peripheral nervous system function in obesity, prediabetes, and T2D is an early event accompanying system-wide metabolic dysregulation (Oort et al. 2008). To our knowledge, our present finding is the first evidence that Sncg expression in skeletal muscle was elevated in high-fat diet-induced obese mice, suggesting that significant attenuation in Sncg expression levels in CC may revive compromised neural signaling other than that in adipose tissue to improve system-wide metabolic dysregulation.
The expression level of Rrad, an inhibitor of voltage-gated calcium channels, was reported to be associated with the accumulation of reactive oxygen species (Halter et al. 2010), and more importantly, Rrad was identified as being overexpressed in the skeletal muscle of T2D patients and diabetic rats (Reynet and Kahn 1993). Another gene that is up-regulated in diabetic patients is Ppp2r3a, which encodes for serine/threonine specific protein phosphatase 2 (also known as protein phosphatase 2A, PP2A) (Højlund et al. 2002; Mumby and Walter 1993) which dephosphorylates a number of enzymes in the insulin signaling cascade believed to regulate glucose transport and glycogen synthesis. In the present study, the HFCC and HFGC diets suppressed the up-regulation of Rrad expression, and the HFGC diet attenuated the increase in Ppp2r3a expression. CA was also reported to lower plasma glucose levels (Jung 2006), from which we may infer that CA which is abundant in CC and GC may contribute to the reduction in Rrad expression. In addition, the lowered levels of Ppp2r3a in the HFGC mice corresponded to the mediated insulin signaling observed from an increase in the tyrosine phosphorylation of IRS-1 and p85/IRS-1 complex.
In much of the world, the rising incidence of obesity demands novel preventive and therapeutic approaches to the treatment of obesity-associated complications—particularly insulin resistance—that lead to T2D and promote cardiovascular disease. Chronic over-nutrition leading to obesity (Esposito 2002; Mohanty et al. 2000) has resulted in elevated levels of inflammatory markers which might interfere with insulin action by suppressing insulin signal transduction, resulting in insulin resistance, which in turn promotes inflammation (Dandona et al. 2001; Aljada et al. 2002). In the present study, coffee intake exerted its anti-inflammatory properties in pathways related to the down-regulation of Atf3, Fos, Socs3, Hspa1a, Hspa1b, and Sncg. Mediated inflammation-associated insulin signaling was also partly reflected in the decreased expressions of Scd1 and Spp1. In addition, the up-regulation of Rrad and Ppp2r3a by the HF diet, which is similar to the up-regulation seen in diabetic patients, was improved by coffee consumption. According to the dose translation from mouse studies to human (Reagan-Shaw et al. 2007), the 2 % coffee supplementation used in this study is equivalent to 4 cups of coffee per day, in a 60-kg human being, a daily dosage which is not difficult to achieve.
This study was partly supported by a grant from the All Japan Coffee Association.
Conflict of interest
All the authors declare no conflict of interest.
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